Written by

Kristen Cramer

June 16, 2025

Edited by

Tamatha Hazen

Fact-checked by

Tara Farmer

Average vitamin D test price

A vitamin D test costs $70 to $100 on average at a lab, depending on the type of test, your location, and your insurance coverage. An at-home vitamin D test kit costs $40 to $100, including the fee to mail the blood sample to a certified lab for processing.

Vitamin D plays a crucial role in bone health, immune function, and overall well-being. A deficiency can lead to a host of health problems, including fatigue, depression, and weakened bones. If you're experiencing symptoms of deficiency or have risk factors, your healthcare provider may recommend a vitamin D test.

Vitamin D test cost factors

Several factors influence how much you'll pay for a vitamin D test:

• Type of test: The most common and accurate test is the 25-hydroxy vitamin D test (25(OH)D), also known as calcidiol. This measures the main circulating form of vitamin D in your blood, providing the best indication of your body's vitamin D stores. A less common test, the 1,25-dihydroxy vitamin D test (1,25(OH)2D), measures the active form and is usually reserved for specific medical conditions.

• Testing facility: Tests conducted at hospitals are often more expensive than those at independent labs or urgent care centers.

• Insurance coverage: Many health insurance plans cover vitamin D testing if a doctor deems it medically necessary. However, insurance may deny coverage if it's considered a routine screening without symptoms or risk factors.

• Geographic location: Testing costs vary significantly by region. Labs in urban areas and states with a higher cost of living typically charge more. Rural areas may have limited testing facilities, potentially affecting prices.

• Additional tests ordered: If your doctor orders multiple blood tests simultaneously, you may benefit from bundled pricing, though this can also increase your total bill.

What is vitamin D and what does it do?

Vitamin D is a fat-soluble vitamin your body produces when your skin is exposed to sunlight. It helps your body absorb calcium from food, supporting strong bones and teeth. Vitamin D also plays an important role in immune function, muscle strength, and cell growth regulation throughout the body.

Why would a doctor prescribe a vitamin D test?

Your doctor may order a vitamin D test if you show symptoms of deficiency or have conditions that affect vitamin D metabolism. Common reasons include:

Symptoms of vitamin D deficiency:

• Bone pain and muscle weakness

• Frequent infections or slow wound healing

• Fatigue and mood changes

• Hair loss or dental problems

High-risk groups:

• People with limited sun exposure

• Individuals with darker skin living in northern climates

• Older adults (over 65)

• People with malabsorption disorders

• Those following strict vegan diets

Medical conditions that require monitoring:

• Osteoporosis or osteopenia

• Chronic kidney disease

• Hyperparathyroidism

• Certain autoimmune conditions

Medication monitoring:

• Patients taking high-dose vitamin D supplements

• Those on medications that affect vitamin D metabolism

What does a vitamin D test involve?

A vitamin D test is a simple blood test and usually involves the following process:

1. Preparation: Generally, a vitamin D test does not require fasting or other special preparation. However, it's always best to confirm with your healthcare provider or the lab.

2. Blood draw: A healthcare professional will draw a blood sample, usually from a vein in your arm. This process typically takes less than five minutes.

3. Lab analysis: The blood sample is sent to a laboratory for analysis to measure the levels of 25(OH)D in your blood.

How long does it take to get vitamin D test results?

The turnaround time for vitamin D test results is generally quick. Most labs provide results within 1 to 3 business days. The results for at-home vitamin D test kits typically take 5 to 10 business days, including shipping time.

Vitamin D test FAQs

What is vitamin D listed as on a blood test?

Vitamin D is typically listed as "25-hydroxyvitamin D" or "25(OH)D" on blood tests. This measures the storage form of vitamin D in your body and is the standard marker used to assess vitamin D status. Some labs may also list it as "calcidiol" or "vitamin D, 25-hydroxy".

Do you need to fast for a vitamin D blood test?

No, fasting is not required for a vitamin D blood test. You can eat and drink normally before this test, as food intake doesn't affect vitamin D levels in your blood. However, always follow any specific instructions given by your healthcare provider or laboratory.